Open Access Highly Accessed Preliminary communication

Exploding vesicles

Ting F Zhu12 and Jack W Szostak1*

1 Howard Hughes Medical Institute, Department of Molecular Biology and the Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA

2 Current address: School of Life Sciences, Biotech Building 4-201, Tsinghua University, Beijing 100084, China

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Journal of Systems Chemistry 2011, 2:4  doi:10.1186/1759-2208-2-4

Published: 1 December 2011

Additional files

Additional file 1:

Additional information. This file includes Methods, Additional Text, and Additional Figures [35-41].

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Open Data

Additional file 2:

Exploding vesicles. This real-time movie shows that oleate vesicles (containing 10 mM HPTS, in 0.2 M Na-bicine, pH 8.5) exploded shortly (~0.5 sec) after being exposed to intense illumination (QuickTime; 9 FPS; 1 MB). Scale bar, 10 μm.

Format: MOV Size: 735KB Download file

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Open Data

Additional file 3:

High-speed movie of an exploding vesicle. This high-speed (played at 1/100 actual speed) movie shows an oleate vesicle (containing 10 mM HPTS, in 0.2 M Na-bicine, pH 8.5) exploding under intense illumination (QuickTime; 15 frames per 10 ms; 4 MB). Scale bar, 10 μm.

Format: MOV Size: 3.9MB Download file

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Open Data

Additional file 4:

Exploding vesicle labeled by a membrane dye. This real-time movie shows an oleate vesicle (containing 10 mM HPTS, in 0.2 M Na-bicine, pH 8.5, labeled by a membrane-localized dye (Rh-DHPE)) that exploded and ruptured its membrane, under intense illumination at the excitation wavelength for HPTS (QuickTime; 9 FPS; 1 MB). Scale bar, 10 μm.

Format: MOV Size: 262KB Download file

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Open Data

Additional file 5:

Explosion of a multilamellar vesicle. This real-time movie shows the gradual rupture of multiple layers of vesicle membranes, starting from the outer layers of an oleate vesicle (containing 10 mM HPTS, in 0.2 M Na-bicine, pH 8.5) and progressing to the inner layers (QuickTime; 9 FPS; 2 MB). Scale bar, 10 μm.

Format: MOV Size: 1.5MB Download file

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Open Data

Additional file 6:

Critical osmotic gradient for membrane rupture. This real-time movie shows a vesicle being diluted into a hypotonic solution through a micropipette. The outer membranes ruptured, and the smaller internal vesicles were released (under low intensity illumination for imaging) (QuickTime; 9 FPS; 2 MB). Scale bar, 10 μm.

Format: MOV Size: 1.5MB Download file

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Open Data

Additional file 7:

Size dependence of vesicle explosions. This real-time movie shows that in a population of polydisperse oleate vesicles (containing 10 mM HPTS, in 0.2 M Na-bicine, pH 8.5), under intense illumination, the larger (> 3 μm in diameter) vesicles explode rapidly while the smaller ones remain intact (QuickTime; 9 FPS; 3 MB). Scale bar, 10 μm.

Format: MOV Size: 2.8MB Download file

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Additional file 8:

Nanoparticle release from exploding vesicle. This real-time movie shows that an oleate vesicle (containing 10 mM HPTS, in 0.2 M Na-bicine, pH 8.5) containing encapsulated biotin-coated fluorescent nanoparticles (40 nm in diameter) exploded shortly after being exposed to intense illumination, releasing a cloud of nanoparticles, which attached to a streptavidin-coated glass surface (QuickTime; 9 FPS; 3 MB). Scale bar, 10 μm.

Format: MOV Size: 3.4MB Download file

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Additional file 9:

Lysosome explosions in adult C. elegans. This real-time movie shows that under intense UV (360 ± 20 nm) illumination, autofluorescent lysosomes explode, releasing the lysosomal contents (QuickTime; 5 FPS; 10 MB). Scale bar, 10 μm.

Format: MOV Size: 10.5MB Download file

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Open Data